Application
Staining of formalin-fixed, paraffin-embedded human placenta sections with 1:500 Monoclonal Anti-Collagen, Type IV, Clone: COL-94 using biotinExtrAvidinR-Peroxidase.Application
Staining of formalin-fixed, paraffin-embedded human tongue sections with 1:500 Monoclonal Anti-Collagen, Type IV, Clone: COL-94 using biotin/ExtrAvidinR-Peroxidase.| Product Name | Collagen IV antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [COL - 94] to Collagen IVCollagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | COL-94 |
| Host Species | Mouse |
| Tested Applications | DotELISAICC/IFIHC-FIHC-PWB |
| Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IHC-P: Use at a dilution of 1/500. ICC/IF: Use at a dilution of 1/500. IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 120070 FLJ22259 MGC42377 1285 P02462 1284 ATS 1286 COL4A5 120130 P08572 COL4A4 1287 CA54 Collagen IV COL4A2 CA44 120131 Q01955 COL4A3 301050 120090 1282 ASLN COL4A1 P29400 P53420 arresten |
|---|---|
| Target | Collagen IV |
| Tissue Specificity | Specifically recognizes native, undenatured, human collagen type IV . Shows no cross reactivity with collagen types I, II, III, V, VI, VII, human vitronectin, fibronectin, or chondroitin sulfate A. B and C. No reactivity with denatured or denatured-reduced collagen in immunoblots. |
| Cellular Localization | Cell surface (Potential). |
Application
Staining of formalin-fixed, paraffin-embedded human placenta sections with 1:500 Monoclonal Anti-Collagen, Type IV, Clone: COL-94 using biotinExtrAvidinR-Peroxidase.
Application
Staining of formalin-fixed, paraffin-embedded human tongue sections with 1:500 Monoclonal Anti-Collagen, Type IV, Clone: COL-94 using biotin/ExtrAvidinR-Peroxidase.| Positive Control | Human tissue |
|---|---|
| Application Notes | Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IHC-P: Use at a dilution of 1/500. ICC/IF: Use at a dilution of 1/500. IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Ascites fluid |
