Application
ELISA analysis of control antigen and antigen using CYP3A4 antibody [3H8].Application
WB analysis of human CYP3A4 (a.a. 243-430) recombinant protein using CYP3A4 antibody [3H8].Application
WB analysis of lysates from (1) untransfected and (2) CYP3A4 (a.a. 243-430)-hIgGFc transfected HEK293 cells using CYP3A4 antibody [3H8].Application
IHC-P analysis of liver cancer tissues using CYP3A4 antibody [3H8].Application
IHC-P analysis of human brain tissues using CYP3A4 antibody [3H8].Application
ICC/IF analysis of HepG2 cells using CYP3A4 antibody [3H8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
FACS analysis of HepG2 cells using CYP3A4 antibody [3H8] (green) or a negative control (red).| Product Name | CYP3A4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3H8] to CYP3A4This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011] |
| Immunogen | The immunogen corresponding to human CYP3A4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CP34 NF-25 CYP3A3 P450C3 CP33 HLP CYPIIIA3 P450PCN1 CYP3A CYPIIIA4 |
|---|---|
| Target | CYP3A4 |
| Molecular Weight(MW) | 57.3 kDa (note) |
Application
ELISA analysis of control antigen and antigen using CYP3A4 antibody [3H8].
Application
WB analysis of human CYP3A4 (a.a. 243-430) recombinant protein using CYP3A4 antibody [3H8].
Application
WB analysis of lysates from (1) untransfected and (2) CYP3A4 (a.a. 243-430)-hIgGFc transfected HEK293 cells using CYP3A4 antibody [3H8].
Application
IHC-P analysis of liver cancer tissues using CYP3A4 antibody [3H8].
Application
IHC-P analysis of human brain tissues using CYP3A4 antibody [3H8].
Application
ICC/IF analysis of HepG2 cells using CYP3A4 antibody [3H8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
FACS analysis of HepG2 cells using CYP3A4 antibody [3H8] (green) or a negative control (red).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
