Application
D-dimer, D-monomer and fibrinogen immunodetection by D-Dimer antibody [DD1] . Lane 1: D-dimer; Lane 2: D-monomer; Lane 3: fibrinogen; Lane 4; D-dimer treated with mercaptoethanolApplication
Titration curves of D-dimer antibody [DD1] . Antigen for coating: 200 ng/well; Black: D-dimer; Green: fibrinogen; Red: D-monomer; Initial antibody concentration: 100 ug/ml| Product Name | D-Dimer antibody [DD1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DD1] to D - DimerFibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream. |
| Immunogen | This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DD1 |
| Host Species | Mouse |
| Tested Applications | ELISAIAWB |
| ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with this antibody as capture and GTX10054 Mouse monoclonal [DD4] to D-Dimer , GTX10055 Mouse monoclonal [DD5] to D-Dimer or GTX10056 Mouse monoclonal [DD6] to D-Dimer as detection. Less than 10ng/ml of D-dimer can be detected using this antibody and HRP labeled GTX10056 [DD6]. To be analyzed in sandwich immunoassay, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | D Dimer, DDimer |
|---|---|
| Target | D-Dimer |
| Tissue Specificity | It is recognized D-dimer and high molecular weight fibrin degradation products with different specificities.No cross-reaction with fibrinogen and D-monomer. |
Application
D-dimer, D-monomer and fibrinogen immunodetection by D-Dimer antibody [DD1] . Lane 1: D-dimer; Lane 2: D-monomer; Lane 3: fibrinogen; Lane 4; D-dimer treated with mercaptoethanol
Application
Titration curves of D-dimer antibody [DD1] . Antigen for coating: 200 ng/well; Black: D-dimer; Green: fibrinogen; Red: D-monomer; Initial antibody concentration: 100 ug/ml| Application Notes | ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with this antibody as capture and GTX10054 Mouse monoclonal [DD4] to D-Dimer , GTX10055 Mouse monoclonal [DD5] to D-Dimer or GTX10056 Mouse monoclonal [DD6] to D-Dimer as detection. Less than 10ng/ml of D-dimer can be detected using this antibody and HRP labeled GTX10056 [DD6]. To be analyzed in sandwich immunoassay, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at 4ºC |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
