| Product Name | D-Dimer antibody [DD4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DD4] to D - DimerFibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream. |
| Immunogen | This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | DD4 |
| Host Species | Mouse |
| Tested Applications | ELISAIAWB |
| ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with GTX10050 Mouse monoclonal [DD1] to D-Dimer , GTX10052 Mouse monoclonal [DD2] to D-Dimer and GTX10053 Mouse monoclonal [DD3] to D-Dimer . To be analyzed in sandwich immunoassay, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more. Because of cross-reaction of available antibodies used for detection in sandwich ELISA (this antibody, GTX10055, and GTX10056) with fibrinogen we strongly recommend the 2 step sandwich ELISA technique. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | D Dimer, DDimer |
|---|---|
| Target | D-Dimer |
| Tissue Specificity | It is recognized D-dimer and high molecular weight fibrin degradation products with different specificities.Cross-reaction with fibrinogen; Low cross-reaction with D-monomer. |
| Application Notes | ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with GTX10050 Mouse monoclonal [DD1] to D-Dimer , GTX10052 Mouse monoclonal [DD2] to D-Dimer and GTX10053 Mouse monoclonal [DD3] to D-Dimer . To be analyzed in sandwich immunoassay, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more. Because of cross-reaction of available antibodies used for detection in sandwich ELISA (this antibody, GTX10055, and GTX10056) with fibrinogen we strongly recommend the 2 step sandwich ELISA technique. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
