Application
Western blot analysis using DDX4 mAb against DDX4-hIgGFc transfected HEK293 cell lysate.Application
Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).Application
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using anti-DDX4 antibody with DAB staining.Application
Figure 2:Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using anti-DDX4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Product Name | DDX4 antibody [2F9H5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2F9H5] to DDX4DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Immunogen | The immunogen corresponding to human DDX4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F9H5 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | VASA |
|---|---|
| Target | DDX4 |
| Molecular Weight(MW) | 76 kDa (note) |
Application
Western blot analysis using DDX4 mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Application
Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using anti-DDX4 antibody with DAB staining.
Application
Figure 2:Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using anti-DDX4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
