Rabbit polyclonal to DesminDesmin is the main intermediate filament in mature skeletal, cardiac, and smooth muscle cells.
Immunogen
Full length protein (Human).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFIHCIHC-FIHC-PWB
For IF: Use at an assay dependent dilution. For IHC-Fr: Use at a dilution of 1:75-1:150. For IHC-P: Use at a dilution of 1:75-1:150 when pretreated with enzymatic or heat mediated antigen retrieval. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins.This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.
Cellular Localization
Cytoplasmic
Application
Application
Application
Positive Control
Heart muscle, smooth and skeletal muscle, leiomyoma and rhabdomyomas
Application Notes
For IF: Use at an assay dependent dilution. For IHC-Fr: Use at a dilution of 1:75-1:150. For IHC-P: Use at a dilution of 1:75-1:150 when pretreated with enzymatic or heat mediated antigen retrieval. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
PBS, pH7.2 containing 0.2% BSA and 0.1% sodium azide