Application
Cardiomyocyte isolated from rat heart stained using Monoclonal Anti-Desmin, clone DE-U-10 (Cat. No. ). The cross-striation corresponding to the Z bands is evident. Some striations are continuously crossing the cell, others show branching that is equivalent to the course of the sarcomeres. Due to the isolation procedure, the cell contains only one membrane of the intercalated disc at both ends that labels for desmin. Nuclei are stained with propidium iodide.| Product Name | Desmin antibody [DE-U-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DE - U - 10] to DesminDesmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle. DE-U-10 labels desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas) (1). |
| Immunogen | Full length native protein (purified) (Pig). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DE-U-10 |
| Host Species | Mouse |
| Tested Applications | Cell CultureFACSICC/IFIHCIHC-FIHC-PWB |
| IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/20. IHC-P: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Monoclonal anti-desmin may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRatCatGoatHamsterLizardRabbitSheepSnakeBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P17661, CSM2, 125660, FLJ39719, FLJ41013, 1674, CSM1, Desmin, FLJ41793, FLJ12025, DES, CMD1I |
|---|---|
| Target | Desmin |
| Tissue Specificity | This antibody is specific for desmin as determined by indirect immunofluorescent staining methods. It specifically stains the wide desmin band of 50,000 to 55,000 molecular weight by immunoblotting methods. |
| Cellular Localization | Cytoplasmic |
Application
Cardiomyocyte isolated from rat heart stained using Monoclonal Anti-Desmin, clone DE-U-10 (Cat. No. ). The cross-striation corresponding to the Z bands is evident. Some striations are continuously crossing the cell, others show branching that is equivalent to the course of the sarcomeres. Due to the isolation procedure, the cell contains only one membrane of the intercalated disc at both ends that labels for desmin. Nuclei are stained with propidium iodide.| Application Notes | IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/20. IHC-P: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Monoclonal anti-desmin may be used for immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 15mM sodium azide |
