Application
IHC Image using - Detection of Dystrophin by IHC in Human Skeletal Muscle| Product Name | Dystrophin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody DystrophinThis antibody with a protein of 427 kD. This antibody is highly specific to dystrophin and shows no cross-reaction with C-protein, a-actin and human muscle spectrin. Dystrophin is a member of spectrin/alpha actinin family of actin-binding protein. This antibody cross reacts with mouse, rat, chicken, Xenopus and Torpedo. |
| Immunogen | BALB/C mice were immunized with acetylcholine receptor enriched membranes and peripheral membrane proteins from Torpedo nobiliana electric organ. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
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| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pke Dystrophin DXS269 DXS272 1756 DXS268 DXS239 DXS270 mdx DXS142 300376 DXS206 DXSmh7 P11532 DXS230 13405 Dmd DXSmh9 Dp427 DXS164 BMD |
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Application
IHC Image using - Detection of Dystrophin by IHC in Human Skeletal Muscle| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
