Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Product Name | E2F1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [6B5] antibody to E2F 1E2F-1-6B5 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. |
| Immunogen | The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6B5 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. Predicted molecular weight: 47 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | E2F1 E2F-1 Q01094 189971 1869 RBP3 RBBP3 RBAP1 E2F 1 |
|---|---|
| Target | E2F1 |
| Tissue Specificity | This antibody recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Positive Control | MCF-7 |
|---|---|
| Application Notes | For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. Predicted molecular weight: 47 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
