Application
IHC Image using - Detection of E2F1 by IHC in Human Tonsil| Product Name | E2F1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [KH95] to E2F1E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F 1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F 1 overexpression can also drive cells into apoptosis. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | KH95 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-P |
| Immunohistochemistry: use at a dilution of 1/25 - 1/50 for 30mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | E2F1 E2F-1 Q01094 189971 1869 RBP3 RBBP3 E2F 1 |
|---|---|
| Target | E2F1 |
| Tissue Specificity | Protein of 60 kDa. |
| Cellular Localization | Nuclear |
Application
IHC Image using - Detection of E2F1 by IHC in Human Tonsil| Positive Control | Tonsil and Breast carcinoma. |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1/25 - 1/50 for 30mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with sodium azide |
