| Product Name | E2F2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CC11] to E2F2E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 2 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides. |
| Immunogen | Recombinant protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | CC11 |
| Host Species | Mouse |
| Tested Applications | GSAIPWB |
| GSA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F2. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | E2F-2 600426 Q14209 E2F2 1870 E2F 2 |
|---|---|
| Target | E2F2 |
| Tissue Specificity | Does not detect the other members of the E2F family. |
| Cellular Localization | Nuclear |
| Application Notes | GSA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F2. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
