| Product Name | E2F4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [WUF3] to E2F4E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F 4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides. |
| Immunogen | Recombinant protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | GSAIPWB |
| GSA: Use at an assay dependent dilution. IP (denatured): Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 µ g/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F4. Detects a band of approximately 50 kDa (for natural molecule, may vary in a recombinant preparation). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | 600659 E2F-4 1874 E2F4 Q16254 E2F 4 |
|---|
| Application Notes | GSA: Use at an assay dependent dilution. IP (denatured): Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 µ g/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F4. Detects a band of approximately 50 kDa (for natural molecule, may vary in a recombinant preparation). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
