Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody diluted at 1:2000.Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody diluted at 1:200.Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody diluted at 1:400.Application
E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. E-cadherin antibody dilution: 1:200.Application
Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody . Western blot analysis was performed using E-Cadherin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 3 μg of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. E-Cadherin antibody diluted at 1:200.Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E-Cadherin antibody diluted at 1:200.Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody .| Product Name | E-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to E-Cadherin (cadherin 1, type 1, E-cadherin (epithelial))This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, BovineDog (80%), Pig (83%), Bovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Arc-1 antibody CD324 antibody CDHE antibody ECAD antibody LCAM antibody UVO antibody CDH1 antibody epithelial cadherin antibody cadherin 1 E-cadherin (epithelial) antibody calcium-dependent adhesion protein epithelial antibody cell-CAM 120/80 a |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (2) |
| Cellular Localization | Cell junction , Cell membrane |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody diluted at 1:2000.
Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody diluted at 1:200.
Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody diluted at 1:400.
Application
E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. E-cadherin antibody dilution: 1:200.
Application
Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody . Western blot analysis was performed using E-Cadherin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 3 μg of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. E-Cadherin antibody diluted at 1:200.
Application
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E-Cadherin antibody diluted at 1:200.
Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody .| Positive Control | MCF-7 , MDA-MB-231 , *HT-29 |
|---|---|
| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
