Application
Western blot analysis of extract from MCF-7 and HT29 cells using E-Cadherin(CD324) Antibody| Product Name | E-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to E-CadherinCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. |
| Immunogen | Peptide sequence around aa.864~868(E-W-G-N-R) derived from Human E-Cadherin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Arc-1 CD324 CDHE ECAD LCAM UVO CDH1 epithelial cadherin cadherin 1 E-cadherin (epithelial) calcium-dependent adhesion protein epithelial cell-CAM 120/80 uvomorulin cadherin-1 CAM 120/80 E-Cadherin cadherin 1 type 1 E-cadherin (epithelial |
|---|---|
| Target | E-Cadherin |
| Molecular Weight(MW) | 135 (note) |
Application
Western blot analysis of extract from MCF-7 and HT29 cells using E-Cadherin(CD324) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
