Application
WB ImageEED antibody [GT671] detects EED protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE EED antibody [GT671] dilution: 1:1000Application
WB ImageEED antibody [GT671] detects EED protein by Western blot analysis. A. 30 µg Rat-2 whole cell lysate/extract 10 % SDS-PAGE EED antibody [GT671] dilution: 1:1000Application
WB ImageSample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 10% SDS PAGE diluted at 1:5000| Product Name | EED antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT671] to EED (embryonic ectoderm development)This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EED. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT671 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Japanese medaka, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (93%), Japanese medaka (95%), Xenopus laevis (96%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HEED antibody WAIT1 antibody EED antibody WD protein associating with integrin cytoplasmic tails 1 antibody polycomb protein EED antibody embryonic ectoderm development antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
WB ImageEED antibody [GT671] detects EED protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE EED antibody [GT671] dilution: 1:1000
Application
WB ImageEED antibody [GT671] detects EED protein by Western blot analysis. A. 30 µg Rat-2 whole cell lysate/extract 10 % SDS-PAGE EED antibody [GT671] dilution: 1:1000
Application
WB ImageSample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 10% SDS PAGE diluted at 1:5000| Positive Control | 293T , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , C2C12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
