Application
IP analysis of Jurkat lysates using control IgG and Eg5 antibody. The precipitates were detected by the same Eg5 antibody.Application
WB analysis of MCF7, 293T and HeLa lysates using Eg5 antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using Eg5 antibody at a dilution of 1:200.| Product Name | Eg5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4H3-1F12] to Eg5This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. |
| Immunogen | Purified recombinant human KIF11 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIF11 kinesin family member 11 MCLMR EG5 KNSL1 HKSP TRIP5 |
|---|---|
| Molecular Weight(MW) | 130kDa (note) |
Application
IP analysis of Jurkat lysates using control IgG and Eg5 antibody. The precipitates were detected by the same Eg5 antibody.
Application
WB analysis of MCF7, 293T and HeLa lysates using Eg5 antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using Eg5 antibody at a dilution of 1:200.| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
