Application
EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer . EGFR antibody [C2C3], C-term dilution: 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using EGFR antibody (Green) at 1:1000 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [C2C3], C-term 5 % SDS-PAGE The immunoprecipitated EGFR protein was detected by EGFR antibody [C2C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. EGFR antibody [C2C3], C-term dilution: 1:500.Application
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | EGFR antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, PigRat (87%), Pig (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERBB antibody ERBB1 antibody HER1 antibody PIG61 antibody mENA antibody EGFR antibody receptor tyrosine-protein kinase erbB-1 antibody cell growth inhibiting protein 40 antibody epidermal growth factor receptor antibody proto-oncogene c-ErbB-1 ant |
|---|---|
| Molecular Weight(MW) | 134 kDa (note) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
Application
EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer . EGFR antibody [C2C3], C-term dilution: 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using EGFR antibody (Green) at 1:1000 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [C2C3], C-term 5 % SDS-PAGE The immunoprecipitated EGFR protein was detected by EGFR antibody [C2C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. EGFR antibody [C2C3], C-term dilution: 1:500.
Application
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , *Beas-2 , *A549 , *SiHa , *Hela , *Huh7 , *MSP |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
