Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody . The precipitated EGFR protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent. A: A549 cell lysate. B: IP by EGFR antibody. 7.5% SDS PAGEApplication
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis. Sample: Two independent paraffin-embedded human non-small cell lung cancer. EGFR antibody [N1-2], N-term diluted at 1:500.Application
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | EGFR antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, ChickenRat (93%), Pig (90%), Chicken (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERBB antibody ERBB1 antibody HER1 antibody PIG61 antibody mENA antibody EGFR antibody receptor tyrosine-protein kinase erbB-1 antibody cell growth inhibiting protein 40 antibody epidermal growth factor receptor antibody proto-oncogene c-ErbB-1 ant |
|---|---|
| Molecular Weight(MW) | 134 kDa (note) (5) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody . The precipitated EGFR protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent. A: A549 cell lysate. B: IP by EGFR antibody. 7.5% SDS PAGE
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis. Sample: Two independent paraffin-embedded human non-small cell lung cancer. EGFR antibody [N1-2], N-term diluted at 1:500.
Application
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , A549 , mouse brain , A549 , H1299 , *MSP |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
