Application
HEK293T cells transfected with either RC200368 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF2S1 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.Application
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.| Product Name | eIF2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3H7] to eIF2 alphaFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| Immunogen | Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H7 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| IHC 1:150 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF2S1 EIF2 EIF-2 EIF-2alpha EIF2A EIF-2A EIF2alpha EIF 2 EIF 2A EIF 2alpha |
|---|---|
| Target | eIF2 alpha |
| Tissue Specificity | is specific for human EIF2S1 (NP_004085). |
Application
HEK293T cells transfected with either RC200368 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF2S1 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
Application
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.| Application Notes | IHC 1:150 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
