Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EIF2S1 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.Application
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-EIF2S1 mouse monoclonal antibody. ( Dilution 1:50)Application
Anti-EIF2S1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with EIF2S1Application
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-EIF2S1 mouse monoclonal antibody. ( Dilution 1:50)| Product Name | eIF2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5D10] to eIF2 alphaFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| Immunogen | Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5D10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF2S1 EIF2 EIF-2 EIF-2alpha EIF2A EIF-2A EIF2alpha EIF 2 EIF 2A EIF 2alpha |
|---|---|
| Target | eIF2 alpha |
| Tissue Specificity | is specific for human EIF2S1 (NP_004085). |
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EIF2S1 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
Application
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-EIF2S1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-EIF2S1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with EIF2S1
Application
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-EIF2S1 mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
