Application
HEK293T cells transfected with either RC213728 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EFNA2 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFNA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFNA2.Application
Anti-EFNA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with EFNA2| Product Name | Ephrin A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [2E6] to Ephrin A2This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2E6 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100,: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEK7L ELF1 EFNA2 LERK6 EPLG6 |
|---|---|
| Target | Ephrin A2 |
| Tissue Specificity | is specific for human EFNA2 (NP_001396). |
Application
HEK293T cells transfected with either RC213728 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EFNA2 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFNA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFNA2.
Application
Anti-EFNA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with EFNA2| Application Notes | ICC/IF 1:100,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
