Application
WB of 0.25 to 20ng of phosphorylated Erk and 0.5-4ug of the non-phosphorylated protein. The Phospho-ERK1/2 antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase.| Product Name | ERK1/2 (phospho Thr202/Tyr204) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ERK1/2 (phospho Thr202/Tyr204)Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine. |
| Immunogen | Synthetic phospho-peptide surrounding Thr202/Tyr204 of human P44MAP kinase |
| Conjugation | Agarose |
| Modification | p-Thr202/Tyr204 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:200~1:1000 ICC/IF:1:50~1:250 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | phospho-Erk1/2 (pThr202/Tyr204) PRKM2 MAPK2 p42-MAPK p38 ERK MAPK3 phospho Erk1/2 (pThr202/Tyr204) P42MAPK ERK2 phosphoErk1/2 (pT202/Y204) p40 PRKM1 phosphoErk1/2 (pThr202/Tyr204) phospho-Erk1/2 (pT202/Y204) MAPK1 p41mapk phospho Erk1/2 (p |
|---|---|
| Target | ERK1/2 (phospho Thr202/Tyr204) |
| Tissue Specificity | This antibody is specific for Erk1/2 (phospho Thr202/Tyr204). |
Application
WB of 0.25 to 20ng of phosphorylated Erk and 0.5-4ug of the non-phosphorylated protein. The Phospho-ERK1/2 antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase.| Application Notes | WB:1:200~1:1000 ICC/IF:1:50~1:250 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. For long term storage, aliquot and freeze at –70°C. Avoid repeated freeze/defrost cycles. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, and 0.02% thimerosal |
