Application
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of| Product Name | ERp57 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MaP.Erp57] to ERp57ERp57, also known as GRP58, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MaP.Erp57 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 0.5 μg/ml. This concentration is sufficient for the detection of ERp57 in 20μg of HeLa heat shocked cell lysate by colorimetric analysis. Predicted molecular weight: 61 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogMonkeyPigHamster |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | 602046 GRP57 2923 GRP58 P30101 ERp60 PDIA3 PIPLC ERp57 P58 HsT17083 PI-PLC ERp61 PI PLC |
|---|---|
| Target | ERp57 |
| Cellular Localization | Endoplasmic reticulum |
Application
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of| Positive Control | HeLa Heat Shocked Cell Lysate |
|---|---|
| Application Notes | IP: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 0.5 μg/ml. This concentration is sufficient for the detection of ERp57 in 20μg of HeLa heat shocked cell lysate by colorimetric analysis. Predicted molecular weight: 61 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 50% Glycerol, 5mM Sodium Phosphate, 25mM Sodium Chloride, 0.05mM PMSF. pH 7.2 |
