| Product Name | FADD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FD19] to FADDFADD (Fas Associated Death Domain) is an apoptosis adapter molecule enabling transduction of the apoptosis signal initiated via the FasL/Fas receptor interaction. The protein contains a C-terminal death domain that interacts with the Fas receptor death domain. The N-terminus contains a death effectors domain (DED, which recruits caspase to the death inducing signaling complex (DISC) and initiates the apoptotic caspase cascade. FADD is implicated in non-apoptotic cellular pathways such as the regulation of cell cycle machinery in T lymphocytes. This is connected to the phosphorylation state of FADD and to the FasL/TRAIL-induced transcriptional activation of c-fos protooncogene. FADD also interacts with the hepatitis C virus core protein in the HEK-293 cell line. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FD19 |
| Host Species | Mouse |
| Tested Applications | DotELISAIP |
| Dot: Use at a concentration of 0.5-1 μg/ml, determined using total cell extracts of A431 cells. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602457 GIG3 Q13158 8772 MORT1 MGC8528 FADD |
|---|---|
| Target | FADD |
| Cellular Localization | Nuclear |
| Application Notes | Dot: Use at a concentration of 0.5-1 μg/ml, determined using total cell extracts of A431 cells. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
