Application
Western blot analysis using FAK mAb against FAK-hIgGFc transfected HEK293 cell lysate.Application
Figure 2:Immunofluorescence analysis of A549 cells using FAK mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Flow cytometric analysis of Raji cells using FAK mAb (green) and negative control (purple).| Product Name | FAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10H7E9] to FAKThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010] |
| Immunogen | The immunogen corresponding to human FAK expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10H7E9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PP125FAK FADK PTK2 P125FAK PPP1R71 FRNK FAK1 |
|---|---|
| Target | FAK |
| Molecular Weight(MW) | 119 kDa (note) |
Application
Western blot analysis using FAK mAb against FAK-hIgGFc transfected HEK293 cell lysate.
Application
Figure 2:Immunofluorescence analysis of A549 cells using FAK mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Flow cytometric analysis of Raji cells using FAK mAb (green) and negative control (purple).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
