Application
Western blot analysis of extracts of various cell lines, using FANCD2 antibody.Application
Immunofluorescence analysis of A549 cell using FANCD2 antibody.Application
Immunofluorescence analysis of MCF7 cell using FANCD2 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using FANCD2 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.| Product Name | FANCD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FANCD2The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human FANCD2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FAD FA D2 Fanconi anemia complementation group D2 FANCD FACD FA4 FAD2 |
|---|---|
| Target | FANCD2 |
Application
Western blot analysis of extracts of various cell lines, using FANCD2 antibody.
Application
Immunofluorescence analysis of A549 cell using FANCD2 antibody.
Application
Immunofluorescence analysis of MCF7 cell using FANCD2 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using FANCD2 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
