Application
Western Blot: FANCD2 Antibody (FI-17) - MCF7 cell line (lane 1) and HeLa whole cell extract (lane 2) using .Application
Western Blot: FANCD2 Antibody (FI-17) - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - 1:1000 LCL 6% SDS-PAGE.| Product Name | FANCD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FI - 17] to FANCD2Fanconi Anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANC D2, targeting nuclear foci containing the BRCA 1 protein. |
| Immunogen | FANCD2 fusion protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FI-17 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000 - 1/2000. Detects a band of approximately 155 and 162 kDa (post-translationally modified form). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FACD FA-D2 2177 FANCD2 FA4 FANCD DKFZp762A223 227646 Q9BXW9 FAD FAD2 FA D2 |
|---|---|
| Target | FANCD2 |
| Tissue Specificity | Specific for human FANCD2. |
Application
Western Blot: FANCD2 Antibody (FI-17) - MCF7 cell line (lane 1) and HeLa whole cell extract (lane 2) using .
Application
Western Blot: FANCD2 Antibody (FI-17) - Expression of FancD2 (mAb) in different cell lines. Proliferating cells were treated with 10-Gy IR. Fifty microgram protein was loaded each lane. Working dilution - 1:1000 LCL 6% SDS-PAGE.| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000 - 1/2000. Detects a band of approximately 155 and 162 kDa (post-translationally modified form). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
