Application
FANCD2 antibody [103] detects FANCD2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCD2 antibody [103] diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Application| Product Name | FANCD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal to human FANCD2 (Fanconi anemia D2) Antibody [Clone 103]Fanconi Anemia Complementation Group D2 (FANCD2) protein is one of at least six factors shown to be involved in the autosomal-recessive cancer-prone disorder, Fanconi Anemia (FA). FA group D has been shown to be comprised of two separate proteins, FANCD1 and FANCD2. Mutations in BRCA2 can cause FANCD1. FANCD2 has been shown to colocalize with BRCA1 in ionizing radiation-induced foci. FANCD2 is involved in genomic resistance to DNA cross-linking reagents, and the arrest of DNA synthesis following exposure to ionizing radiation. FANCD2 has been shown to directly interact with NBS1 |
| Immunogen | Human FANCD2 fusion protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 103 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| For WB: Use at a dilution of 1:500-1:3000. For ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FACD FA-D2 2177 FANCD2 FA4 FANCD DKFZp762A223 227646 Q9BXW9 FAD FAD2 FA D2 |
|---|---|
| Target | FANCD2 |
| Cellular Localization | Nuclear |
Application
FANCD2 antibody [103] detects FANCD2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCD2 antibody [103] diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Application| Positive Control | 293T , HeLa |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:3000. For ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
