Application
Western blot analysis of FEN1 expression in Raji (A) and NIH-3T3 (B) whole cell lysates.| Product Name | FEN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antiserum to human FEN1FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease when it cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems. |
| Immunogen | Recombinant full-length human FEN-1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:2,500. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RAD2, P39748, FEN-1, 600393, MF1, FEN1, 2237, FEN 1 |
|---|---|
| Target | FEN1 |
| Cellular Localization | Nuclear |
Application
Western blot analysis of FEN1 expression in Raji (A) and NIH-3T3 (B) whole cell lysates.| Positive Control | HeLa, Raji, 3T3 |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:2,500. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
