Mouse polyclonal antiserum to human FEN1FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42a protein that functions as an endonuclease when it cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems.
Immunogen
Recombinant full-length human FEN-1 protein.
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Mouse
Tested Applications
WB
For WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the researcher.
Detecion of human FEN1 protein in HeLa whole cell lysate using GeneTex polyclonal antiserum
Positive Control
MCF7
Application Notes
For WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer
Phosphate-buffered saline, pH 7.4, containing no preservatives