Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7] antibody at 1:200 dilution.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | FEN1 antibody [4E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4E7] FEN1FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems. |
| Immunogen | Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RAD2, P39748, FEN-1, 600393, MF1, FEN1, 2237, FEN 1 |
|---|---|
| Target | FEN1 |
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7] antibody at 1:200 dilution.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | MCF7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
