Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.Application
Flow cytometric Analysis of Jurkat cells, using anti-FHL1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.| Product Name | FHL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1D6] to FHL1May have an involvement in muscle development or hypertrophy. |
| Immunogen | Full length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D6 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Flow Cytometry 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KYO-T KYOT FHL1B FHL1 bA535K18.1 SLIM1 MGC111107 KYO T |
|---|---|
| Target | FHL1 |
| Tissue Specificity | is specific for human FHL1 (NP_001440). |
| Cellular Localization | Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes. |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Application
Flow cytometric Analysis of Jurkat cells, using anti-FHL1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.| Application Notes | Flow Cytometry 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
