Application
Detection of fibrinogen by fibrinogen antibodies in Western blotting (non-reducing conditions). Lane 1: Fibrinogen antibody [1F3] ; Lane 2: Fibrinogen antibody [15H12]| Product Name | Fibrinogen antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1F3] to FibrinogenFibrinogen is the main protein of blood coagulation system. It is a large protein (MW 340 kDa) and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1-16 amino acids) and B (1-17 amino acids) are released by thrombin from the N-terminal parts of alpha- and beta-chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. |
| Immunogen | Fibrin degradation products |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F3 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with GTX10067 Mouse monoclonal [27C8] to Fibrinogen as detection and GTX10068 Mouse monoclonal [40F11] to Fibrinogen as capture. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | FGA 134830 300163 MGC119423 MGC120405 MGC104327 KYO-T 105200 KYOT P02675 Fibrinogen SLIM1 MGC119425 MGC111107 2244 2243 2273 FHL1B Fib2 FGB FHL1 Q13642 P02671 MGC119422 bA535K18.1 KYO T |
|---|---|
| Target | Fibrinogen |
| Tissue Specificity | Fibrinogen, Fibrin degradation products |
| Cellular Localization | Secreted |
Application
Detection of fibrinogen by fibrinogen antibodies in Western blotting (non-reducing conditions). Lane 1: Fibrinogen antibody [1F3] ; Lane 2: Fibrinogen antibody [15H12]| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Can be paired for ELISA with GTX10067 Mouse monoclonal [27C8] to Fibrinogen as detection and GTX10068 Mouse monoclonal [40F11] to Fibrinogen as capture. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing 0.1% sodium azide |
