Application
WB analysis of human Fibrinogen gamma recombinant protein (aa. 210-437) using Fibrinogen gamma antibody [4H9].Application
ELISA analysis of control antigen (100ng) and antigen using Fibrinogen gamma antibody [4H9].Application
FACS analysis of HepG2 cells using Fibrinogen gamma antibody [4H9] (blue) and negative control (red).Application
ICC/IF analysis of 3T3-L1 cells using Fibrinogen gamma antibody [4H9] (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
IHC-P analysis of cerebellum tissues using Fibrinogen gamma antibody [4H9].Application
IHC-P analysis of liver cancer tissues using Fibrinogen gamma antibody [4H9].| Product Name | Fibrinogen gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4H9] to Fibrinogen gammaThe protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 210 and 437 of human FGG. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4H9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FGG |
|---|---|
| Target | Fibrinogen gamma |
| Molecular Weight(MW) | 52 kDa (note) |
Application
WB analysis of human Fibrinogen gamma recombinant protein (aa. 210-437) using Fibrinogen gamma antibody [4H9].
Application
ELISA analysis of control antigen (100ng) and antigen using Fibrinogen gamma antibody [4H9].
Application
FACS analysis of HepG2 cells using Fibrinogen gamma antibody [4H9] (blue) and negative control (red).
Application
ICC/IF analysis of 3T3-L1 cells using Fibrinogen gamma antibody [4H9] (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
IHC-P analysis of cerebellum tissues using Fibrinogen gamma antibody [4H9].
Application
IHC-P analysis of liver cancer tissues using Fibrinogen gamma antibody [4H9].| Application Notes | Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
