Application
Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis. A. 30 µg NIH-3T3 whole cell extract 5% SDS-PAGE Filamin B antibody [N1], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Filamin B antibody [N1], N-term diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: U87-MG 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Filamin B antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Filamin B (filamin B, beta)This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, BovineRat (98%), Chicken (97%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABP-278 antibody ABP-280 antibody AOI antibody DKFZp686A1668 antibody DKFZp686O033 antibody FH1 antibody FLN1L antibody LRS1 antibody SCT antibody TABP antibody TAP antibody FLNB antibody thyroid autoantigen antibody ABP-280 homolog antibody |
|---|---|
| Molecular Weight(MW) | 278 kDa (note) (4) |
| Cellular Localization | Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm |
Application
Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis. A. 30 µg NIH-3T3 whole cell extract 5% SDS-PAGE Filamin B antibody [N1], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Filamin B antibody [N1], N-term diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , U87-MG , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
