Application
Western blot analysis of FKBP12 (arrow) using rabbit polyclonal FKBP12 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FKBP12 gene (Lane 2)Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FKBP12 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | FKBP12 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to FKBP12 (Internal)The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK56 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 51~81 amino acids from the Center region of human FKBP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | FK506binding protein1A FKBP-1A 12 kDa FKBP FKBP-12 PPIase FKBP1A Peptidylprolyl cistrans isomerase FKBP1A FK506-binding protein 1A FKBP1A Immunophilin FKBP12 FK506binding protein 1A EC5.2.1.8 Peptidyl-prolyl cis-trans isomerase FKBP1A FKBP1 FK |
|---|---|
| Target | FKBP12 |
| Molecular Weight(MW) | 11.951 kDa (note) |
Application
Western blot analysis of FKBP12 (arrow) using rabbit polyclonal FKBP12 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FKBP12 gene (Lane 2)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FKBP12 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
