Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with FLAP antibody diluted at 1:500.Application
FLAP antibody detects FLAP protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. FLAP antibody dilution: 1:500.Application
FLAP antibody detects FLAP protein at cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. FLAP antibody dilution: 1:500.| Product Name | FLAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FLAP (arachidonate 5-lipoxygenase-activating protein)This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FLAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Pig, Chicken, Rhesus Monkey, BovineHuman (100%), Pig (91%), Chicken (81%), Rhesus Monkey (97%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | arachidonate 5-lipoxygenase-activating protein ALOX5AP |
|---|---|
| Target | FLAP |
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Nucleus membrane , Endoplasmic reticulum membrane |
Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with FLAP antibody diluted at 1:500.
Application
FLAP antibody detects FLAP protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. FLAP antibody dilution: 1:500.
Application
FLAP antibody detects FLAP protein at cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. FLAP antibody dilution: 1:500.| Positive Control | rat spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
