Application
WB analysis of lysates from mouse kidney, MCF7 and rat spleen (40ug per lane) using FLIP antibody at a dilution of 1:500.| Product Name | FLIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5D8] to FLIPThe protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] |
| Immunogen | Recombinant human FLIP (1-376aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 5D8 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | I-FLICE CLARP CASP8AP1 CASP8 and FADD-like apoptosis regulator c-FLIPR CASH c-FLIPL c-FLIPS c-FLIP Casper MRIT FLAME1 FLAME |
|---|---|
| Target | FLIP |
| Light Chain Type | kappa |
Application
WB analysis of lysates from mouse kidney, MCF7 and rat spleen (40ug per lane) using FLIP antibody at a dilution of 1:500.| Application Notes | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
