Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
Sample (50 µg of whole cell lysate) A: rat brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody dilution: 1:500.Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD65 (glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa))This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Dog, Pig, Rhesus Monkey, BovineZebrafish (86%), Dog (96%), Pig (95%), Rhesus Monkey (100%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GAD65 antibody MGC161605 antibody MGC161607 antibody GAD2 antibody 65 kDa glutamic acid decarboxylase antibody glutamate decarboxylase 65 kDa isoform antibody GAD-65 antibody glutamate decarboxylase 2 antibody Glutamate decarboxylase-2 (pancreas) a |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (3) |
| Cellular Localization | Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane , Golgi apparatus membrane |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
Sample (50 µg of whole cell lysate) A: rat brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody dilution: 1:500.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
