Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000Application
GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term diluted at 1:20000.Application
GAD65 antibody [C2C3], C-term detects GAD65 protein expression by immunofluorescent analysis. Sample: Cultured Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GAD65 protein stained by GAD65 antibody [C2C3], C-term diluted at 1:250. Blue: Fluoroshield with DAPI ..Application
Sample (10 ug of whole cell lysate) A: rat brain 7.5% SDS PAGE diluted at 1:10000Application
GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody [C2C3], C-term dilution: 1:500.| Product Name | GAD65 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD65 (glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa))This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GAD65 antibody MGC161605 antibody MGC161607 antibody GAD2 antibody 65 kDa glutamic acid decarboxylase antibody glutamate decarboxylase 65 kDa isoform antibody GAD-65 antibody glutamate decarboxylase 2 antibody Glutamate decarboxylase-2 (pancreas) a |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane , Golgi apparatus membrane |
Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000
Application
GAD65 antibody [C2C3], C-term detects GAD65 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: GAD65 protein stained by GAD65 antibody [C2C3], C-term diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody [C2C3], C-term diluted at 1:20000.
Application
GAD65 antibody [C2C3], C-term detects GAD65 protein expression by immunofluorescent analysis. Sample: Cultured Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GAD65 protein stained by GAD65 antibody [C2C3], C-term diluted at 1:250. Blue: Fluoroshield with DAPI ..
Application
Sample (10 ug of whole cell lysate) A: rat brain 7.5% SDS PAGE diluted at 1:10000
Application
GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody [C2C3], C-term dilution: 1:500.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
