Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:5000.Application
GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: GAD65 protein stained by GAD65 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .| Product Name | GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD65 (glutamic acid decarboxylase 2)Catalyzes the production of GABA. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human GAD65. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Rhesus MonkeyMouse (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 6330404F12Rik antibody GAD(65) antibody GAD65 antibody GAD65 antibody Gad2 antibody glutamic acid decarboxylase 2 antibody |
|---|---|
| Target | GAD65 |
| Molecular Weight(MW) | 65 kDa (note) |
| Cellular Localization | Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane; Lipid-anchor , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse |
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:5000.
Application
GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: GAD65 protein stained by GAD65 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .| Positive Control | APRE-19 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
