Application
Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10 , Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).| Product Name | Glucagon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [K79bB10] to GlucagonGlucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogenesis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide reside almost exclusively with the existence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as an cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in quantification of the hormone. |
| Immunogen | Polymerized porcine glucagon |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | K79bB10 |
| Host Species | Mouse |
| Tested Applications | DotICC/IFIHCRIA |
| Dot: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution, stains Bouin-fixed and formalin-fixed, paraffin-embedded pancreatic tissue sections. Reacts with pancreatic glucagon. IHC: Use at an assay dependent dilution, weakly cross-reacts with gut glucagon (enteroglucagon). RIA: Use at an assay dependent dilution, affinity constant of 6.1 x 10^8 L/M, reacts with pancreatic glucagon. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseDogPigRatCatGuinea PigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GLP1 2641 GRPP GCG P01275 Glucagon GLP2 138030 |
|---|---|
| Target | Glucagon |
| Cellular Localization | Secreted |
Application
Mouse pancreatic islet alpha cells were stained using Monoclonal Anti-Glucagon, clone no. K79bB10 , Anti-Mouse IgG-peroxidase and AEC substrate (red). The tissue was counterstained with hematoxylin (blue).| Positive Control | Pancreatic glucagon for RIA and immunocytochemistry |
|---|---|
| Application Notes | Dot: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution, stains Bouin-fixed and formalin-fixed, paraffin-embedded pancreatic tissue sections. Reacts with pancreatic glucagon. IHC: Use at an assay dependent dilution, weakly cross-reacts with gut glucagon (enteroglucagon). RIA: Use at an assay dependent dilution, affinity constant of 6.1 x 10^8 L/M, reacts with pancreatic glucagon. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. For prolonged storage, freeze in working aliquots. Avoid repeated freezing and thawing. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
