Application
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).Application
Western blot analysis of Grp75/Mortalin, mAb (30A5) : Lane 1: MW marker Lane 2: HeLa (heat shocked) Lane 3: L-929 (heat shocked) Lane 4: CHO -K1 (heat shocked) Lane 5: GPC-16 (heat shocked) Lane 6: PC-12 (heat shocked).| Product Name | Grp75 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [30A5] to Grp75In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATPin vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75 kDa protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to function as molecular chaperones, assisting in the folding of other proteins in various intracellular compartments. Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone. |
| Immunogen | Grp75 isolated from human Molt4 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 30A5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| Immunohistochemistry (1:50) Western Blot (1:1'000, colorimetric) Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | HumanMouseDogMonkeyPigRatGuinea PigHamsterRabbitSheepXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 600548 MGC4500 MTHSP75 GRP75 mot-2 PBP74 MOT 3313 CSA MOT2 HSPA9 HSPA9B P38646 mot 2 |
|---|---|
| Target | Grp75 |
| Tissue Specificity | This antibody detects an ~75 kDa protein, corresponding to the apparent molecular mass of Glucose Regulated Protein 75 (Grp75) on SDS-PAGE immunoblots. |
| Cellular Localization | Mitochondrial |
Application
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).
Application
Western blot analysis of Grp75/Mortalin, mAb (30A5) : Lane 1: MW marker Lane 2: HeLa (heat shocked) Lane 3: L-929 (heat shocked) Lane 4: CHO -K1 (heat shocked) Lane 5: GPC-16 (heat shocked) Lane 6: PC-12 (heat shocked).| Positive Control | HeLa Cell Lysate (Heat Shocked) |
|---|---|
| Application Notes | Immunohistochemistry (1:50) Western Blot (1:1'000, colorimetric) Optimal conditions must be determined individually for each application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None; Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
