Application
Anti-HSPA9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSPA9Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using antiHSPA9 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9.Application
Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody .Application
Flow cytometric analysis of Hela cells, using anti-HSPA9 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-HSPA9 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).| Product Name | Grp75 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9F8] to Grp75Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. |
| Immunogen | Protein expressed in 293T cell transfected with human Grp75 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 9F8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:2000. For ICC/IF: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:50. For FACS: Use at a dilution of 1:100. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GRP75 mot-2 PBP74 HSPA9 MGC4500 MTHSP75 MOT CSA MOT2 mot 2 |
|---|---|
| Target | Grp75 |
| Tissue Specificity | is specific for human Grp75. |
Application
Anti-HSPA9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSPA9
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using antiHSPA9 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using antiHSPA9mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9.
Application
Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody .
Application
Flow cytometric analysis of Hela cells, using anti-HSPA9 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-HSPA9 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:2000. For ICC/IF: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:50. For FACS: Use at a dilution of 1:100. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
