| Product Name | Haptoglobin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HG - 36] to HaptoglobinHaptoglobin combines with free plasma hemoglobin, prevent loss of iron through the kidneys and protect the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. |
| Immunogen | Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HG-36 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| ELISA: Use at a dilution of 1/4000. The minimum antibody titer of 1/4000 was determined by indirect ELISA using 10 μg/ml human haptoglobin for coating the microtiter plate. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hp2-alpha 140100 hp2alpha Haptoglobin MGC111141 P00738 3240 HP hp2 alpha |
|---|---|
| Target | Haptoglobin |
| Tissue Specificity | This antibody reacts with the three types of human haptoglobin (types 1-1, 2-1, and 2-2). In an ELISA, the antibody recognizes human haptoglobin as the purified antigen, in human serum, when complexed with human hemoglobin or captured by antibodies. In a competitive ELISA it does not cross react with human IgG, transferrin, C-reactive protein, alpha 1 acid glycoprotein, albumin or hemoglobin, nor with bovine, goat or sheep serum. The antibody reacts against native and denatured (non-reduced) human haptoglobin in an immunoblot or dot blot but fails to recognize the different haptoglobin types under reducing conditions. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at a dilution of 1/4000. The minimum antibody titer of 1/4000 was determined by indirect ELISA using 10 μg/ml human haptoglobin for coating the microtiter plate. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
