Application
Rabbit Anti-HDAC4 (ML-19) was used to immunoprecipitate HDAC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-HDAC4 (ML-19) . Lanes 1. 10 μg antibody 2. 5 μg antibody 3. Negative control: No cell extract 4. Positive control: HeLa whole cell extract| Product Name | HDAC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HDAC4Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. |
| Immunogen | Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| IF: Use at a dilution of 1/250, this is determined by indirect immunofluorescence using HEK 293T cells expressing recombinant mouse HDAC4IP: Use at an assay dependent dilution. 10-20 μg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 10^7 cells. WB: Use at an assay dependent dilution. Predicted molecular weight: 140 kDa. A minimum working dilution of 1/1000 is determined by blotting using whole extracts of mouse NIH-3T3 cells. A minimum working dilution of 1/500 is determined by blotting using whole extracts of rat brain. Additional weak bands may be detected when blotting using different extract preparations. Detection of HDAC4 by blotting is specifically inhibited with the immunizing peptide. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 605314, HD4, HDAC-A, HDAC4, KIAA0288, 9759, P56524, HA6116, HDACA, HDAC A |
|---|---|
| Target | HDAC4 |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Rabbit Anti-HDAC4 (ML-19) was used to immunoprecipitate HDAC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-HDAC4 (ML-19) . Lanes 1. 10 μg antibody 2. 5 μg antibody 3. Negative control: No cell extract 4. Positive control: HeLa whole cell extract| Positive Control | Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation) |
|---|---|
| Application Notes | IF: Use at a dilution of 1/250, this is determined by indirect immunofluorescence using HEK 293T cells expressing recombinant mouse HDAC4IP: Use at an assay dependent dilution. 10-20 μg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 10^7 cells. WB: Use at an assay dependent dilution. Predicted molecular weight: 140 kDa. A minimum working dilution of 1/1000 is determined by blotting using whole extracts of mouse NIH-3T3 cells. A minimum working dilution of 1/500 is determined by blotting using whole extracts of rat brain. Additional weak bands may be detected when blotting using different extract preparations. Detection of HDAC4 by blotting is specifically inhibited with the immunizing peptide. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
