Application
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Histone Deacetylase 4 (HDAC4) . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HeLa 2. NIH-3T3 3. P19 4. Jurkat 5. Rat brain 6. PC12| Product Name | HDAC4 antibody [HDAC-144] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HDAC - 144] to HDAC4Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. |
| Immunogen | Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | HDAC-144 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml, this concentration is recommended using total cell extracts of NIH3T3 fibroblast cells. Predicted molecular weight: 140 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 605314, HD4, HDAC-A, HDAC4, KIAA0288, 9759, P56524, HA6116, HDACA, HDAC A |
|---|---|
| Target | HDAC4 |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Histone Deacetylase 4 (HDAC4) . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HeLa 2. NIH-3T3 3. P19 4. Jurkat 5. Rat brain 6. PC12| Positive Control | Total cell extracts of NIH3T3 fibroblast cells. |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml, this concentration is recommended using total cell extracts of NIH3T3 fibroblast cells. Predicted molecular weight: 140 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
