Application
WB analysis of MCF7 lysate (40ug) using HEXA antibody at a dilution of 1:3,000.| Product Name | HEXA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT20F1] to HEXAThis gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq, Jul 2009] |
| Immunogen | Recombinant human HEXA (89-529aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AT20F1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:3000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TSD hexosaminidase A (alpha polypeptide) |
|---|---|
| Target | HEXA |
| Light Chain Type | lambda |
Application
WB analysis of MCF7 lysate (40ug) using HEXA antibody at a dilution of 1:3,000.| Application Notes | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:3000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide. |
