Application
Histone H2B antibody detects Histone H2B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted by 1:1000.Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000.| Product Name | Histone H2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H2B (histone cluster 2, H2be)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq] |
| Immunogen | Full length human Histone H2B Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2BFQ histone cluster 2 H2be H2BGL105 H2BQ GL105 H2B HIST2H2BE H2B.1 |
|---|---|
| Target | Histone H2B |
| Molecular Weight(MW) | 14 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H2B antibody detects Histone H2B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted by 1:1000.
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000.| Positive Control | HeLa , HeLa nuclear extract , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
