Application
Histone H3K27ac (acetyl Lys27) antibody [GT261] detects Histone H3K27ac (acetyl Lys27) protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3K27ac (acetyl Lys27) antibody [GT261] diluted at 1:200.Application
Histone H3K27ac (acetyl Lys27) antibody [GT261] detects Histone H3K27ac (acetyl Lys27) protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3K27ac (acetyl Lys27) antibody [GT261] diluted at 1:200.Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] diluted at 1:1000.| Product Name | Histone H3K27ac (acetyl Lys27) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT261] to Histone H3K27ac (acetyl Lys27)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K27ac (acetyl Lys27). The exact sequence is proprietary. |
| Modification | acetyl Lys27 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT261 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3/A antibody H3FA antibody HIST1H3A antibody Histone H3 (acetyl Lys27) antibody [GT261] antibody Histone H3 antibody histone cluster 1 H3a antibody |
|---|---|
| Target | Histone H3K27ac (acetyl Lys27) |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3K27ac (acetyl Lys27) antibody [GT261] detects Histone H3K27ac (acetyl Lys27) protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3K27ac (acetyl Lys27) antibody [GT261] diluted at 1:200.
Application
Histone H3K27ac (acetyl Lys27) antibody [GT261] detects Histone H3K27ac (acetyl Lys27) protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3K27ac (acetyl Lys27) antibody [GT261] diluted at 1:200.
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] diluted at 1:1000.| Positive Control | HeLa , HeLa(0.4 µM Trichostatin A treatment for 18 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
